Figure 3.

Activation of split functionalities during reassociation of RNA nanocubes 3′-side decorated with six RNA–DNA (in blue) hybrids (carrying six Dicer substrate RNA antisense) with six cognate hybrids (carrying Dicer substrate RNA senses). (a) Schematics of reassociation and activation of FRET and RNAi. (b) Assembled RNA cubes were analyzed by total SYBR Gold staining native PAGE and DLS experiments. (c) FRET time traces during reassociation of fluorescently labeled cubes and hybrids labeled with Alexa 488 and Alexa 546. (d) GFP knockdown was quantified through flow cytometry. Please note that the individual hybrids and RNA nanocubes decorated with hybrids cause no decrease in eGFP production.