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. 2014 Dec 18;9(1):251–259. doi: 10.1021/nn504508s

Figure 4.

Figure 4

Activation of different split functionalities during reassociation of DNA nanocubes (in blue) 3′-side decorated with six RNA–DNA hybrids (carrying six DS RNA antisenses, in red) with six cognate hybrids (carrying DS RNA senses). (a) Schematics of reassociation and activation of FRET and RNAi. (b) The formation of DNA cubes was confirmed by total SYBR Gold staining native PAGE and DLS experiments. (c) FRET time traces during reassociation of fluorescently labeled cubes and hybrids labeled with Alexa 546 and Alexa 488. (d) FRET experiments: cells were cotransfected with cubes and cognate hybrids labeled with Alexa 546 and Alexa 488 and images were taken on the next day. (e) GFP knockdown was measured via flow cytometry. Please note that the individual hybrids and DNA nanocubes decorated with hybrids cause no decrease in eGFP expression. Image numbers in (d) correspond to differential interference contrast (DIC) images (1), Alexa 488 emission (2), Alexa 546 emission (3), bleed-through corrected FRET image (4), 3D chart representation of zoomed fragment indicated by a white box of bleed-through corrected FRET image with the white dot indicating the correspondence (5).