Table 1. Relative Differences for RNA–RNA, RNA–DNA, and DNA–RNA Nanoparticles Described in This Work.
functional nanoparticle | processed by Dicer | stable in human serum | activates type I IFNs | activates pro-inflammatory cytokine | activates RNAi |
---|---|---|---|---|---|
RNA–RNA nanocube | YES | NO | YES | YES | YES |
RNA–DNA nanocube | NO | NO | YES | YES | NOa |
DNA–RNA nanocube | NO | YES | NO | NO | NOa |
Cognate RNA–DNA hybrids are required for activation of RNAi during intracellular reassociation.