Figure 9.

Low AMPK activity and diminished expression of UCP2 and SIRT3 in STC1 KO kidneys. Total kidney lysates were obtained 72 hours after I/R or sham surgery; Western blots were reacted with anti-STC1, anti-pAMPKα1,α2, anti-AMPKα1,α2, and anti-actin. Kidney mitochondrial lysates were obtained 72 hours after I/R or sham surgery; Western blots were reacted with anti-UCP2, anti-SIRT3, and anti–F1-ATPase. Bar graphs represent the mean±SEM of data obtained from three mice per group. Sh, sham; tAMPK, total AMPK. *P<0.05 versus WT sham.