Table 1.
Antibodies used for Western blotting and immunofluorescence
| Antibody | Host | Dilution | Source | Reference | |
|---|---|---|---|---|---|
| Western Blotting | Immunofluorescence | ||||
| ROMK | Rabbit | 1:800 | 1:4000 | Alomone | — |
| αENAC | Rabbit | 1:5000 | 1:5000 | J. Loffing | 35 |
| βENaC | Rabbit | 1:10,000 | 1:40,000 | J. Loffing | 61 |
| γENaC | Rabbit | 1:10,000 | 1:40,000 | J. Loffing | 61 |
| NCC | Rabbit | 1:800035 | 1:10,00064 | J. Loffing | 35,64 |
| pNCC-T53 | Rabbit | 1:1000 | R. Fenton | 65 | |
| pNCC-T58 | Rabbit | 1:2000 | R. Fenton | 65 | |
| pNCC-S89 | Rabbit | 1:4000 | J. Loffing | 35 | |
| Calbindin D28k | Mouse | 1:10,000 | Swant (Switzerland) | — | |
| β-actin | Rabbit | 1:10,000 | Sigma | — | |