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Irreversibility of GRX-1 inhibition by 2-AAPA. GRX-1 was incubated with 2-AAPA 1 mM for 1 hour to achieve complete inhibition. Then the solution was transferred to a DisPoDialyzer and dialyzed in Tris buffer for 4 hours. No enzyme activity returned in the 2-AAPA sample over a 4 hour period. The graph shows a representative plot from one of triplicate experiments. ◆, control; ■, 2-AAPA treated.
