FIGURE 2:

Effects of altered SP1 expression on MDK expression in human glioma cell lines. (A) SP1 gene silencing of glioma cell lines U87 and U251 was conducted by lentivirus-delivered shRNA. Cell lysates were subjected to Western blot and RT-PCR. (B) SP1 overexpression and control A172 cell lines were established using lentivirus. MDK and SP1 expression were determined by Western blot and RT-PCR. Data are representative of three separate experiments.