TABLE 1:
S. pombe strains used in this study. Continued
| Strain | Genotype | Source/reference |
|---|---|---|
| JW81 | h− ade6-M210 leu1-32 ura4-D18 | Wu et al., 2003 |
| JW404 | h+ art1-s34-F3 leu1-32 ura4-D18 | Wu et al., 2010 |
| JW739 | h− ade6-M216 leu1-32 ura4-D18 | Lab stock |
| JW797 | h− sad1-CFP-kanMX6 ade6-M210 leu1-32 ura4-D18 | Wu et al., 2003 |
| JW1105 | h+ rgf3-mEGFP-kanMX6 ade6-M210 leu1-32 ura4-D18 | Wu et al., 2010 |
| JW2245 | h+ rgf3-mECitrine-kanMX6 ade6-M210 leu1-32 ura4-D18 | Lab stock |
| JW2421 | h+ rgf3-13Myc-hphMX6 ade6-M210 leu1-32 ura4-D18 | Lab stock |
| JW2543 | h− art1Δ::ura4+ ade6-M216 leu1-32 ura4-D18 | This study |
| JW2674 | h− art1-mECitrine-kanMX6 ade6-210 ura4-D18 leu1-32 | This study |
| JW2694 | art1-mECitrine-kanMX6 sad1-mCFP-kanMX6 ade6-M210 ura4-D18 leu1-32 | This study |
| JW2696 | h− art1-2YFP-kanMX6 rgf3-13Myc-hphMX6 ade6 leu1-32 ura4-D18 | This study |
| JW2748 | h+ rgf3-mECitrine-kanMX6 sad1-CFP-kanMX6 ade6-M210 leu1-32 ura4-D18 | This study |
| JW3055 | h− bgs1Δ::ura4+ Pbgs1-GFP-bgs1-leu1+ leu1-32 ura4-D18 | Cortes et al., 2012 |
| JW3313 | h− leu1-32-kanMX6-3nmt1-mEGFP rlc1-tdTomato-natMX6 ade6-M210 leu1-32 ura4-D18 | This study |
| JW3344 | h− art1(Δ287-483)-mECitrine-kanMX6 ade6-210 leu1-32 ura4-D18 | This study |
| JW3345 | h− art1(Δ429-483)-mECitrine-kanMX6 ade6-210 leu1-32 ura4-D18 | This study |
| JW3351 | art1-mECitrine-kanMX6 kanMX6-Prgf3-N-degron-HA-rgf3 sad1-mCFP-kanMX6 ade6 leu1-32 ura4-D18 | This study |
| JW3381 | h− art1-Stag-kanMX6 ade6-210 leu1-32 ura4-D18 | This study |
| JW3395 | art1Δ::ura4+ leu1-32-kanMX6-3nmt1-mEGFP rlc1-tdTomato-natMX6 ade6 leu1-32 ura4-D18 | This study |
| JW3563 | h− art1Δ::kanMX6 ade6-210 leu1-32 ura4-D18 | This study |
| JW3632 | h− art1Δ::kanMX6 ade6-210 leu1-32 ura4-D18 + pUR19 | This study |
| JW3633 | h− art1Δ::kanMX6 ade6-210 leu1-32 ura4-D18 + pUR19-Rho1 | This study |
| JW3675 | art1(Δ287-428)-mECitrine-kanMX6 ade6-210 leu1-32 ura4-D18 | This study |
| JW3711 | art1-2YFP-kanMX6 ade6 leu1-32 ura4-D18 | This study |
| JW3795 | art1(Δ1-286)-mECitrine-kanMX6 ade6-210 leu1-32 ura4-D18 | This study |
| JW3868 | art1Δ::ura4+ kanMX6-3nmt1-rgf3 ade6 leu1-32 ura4-D18 | This study |
| JW4427 | art1-mECitrine-kanMX6 kanMX6-Pmyo2-mCFP-myo2 ade6-M210 leu1-32 ura4-D18 | This study |
| JW4816 | art1Δ::ura4+ rgf3-13Myc-hphMX6 ade6 leu1-32 ura4-D18 | This study |
| JW4889 | rgf3-mECitrine-kanMX6 kanMX6-P3nmt1-art1 ade6 leu1-32 ura4-D18 | This study |
| JW5129 | rgf3-13Myc-hphMX6 kanMX6-P3nmt1-mECitrine-art1 ade6 leu1-32 ura4-D18 | This study |
| JW5232 | rgf3-mECitrine-kanMX6 art1Δ::ura4+ sad1-mCFP-kanMX6 ade6 leu1-32 ura4-D18 | This study |
| JW5593 | h− leu1::kanMX6-P3nmt1-pkc1(HR1-C2)-mECitrine ade6-M210 leu1-32 ura4-D18 | This study |
| JW5712 | art1-mECitrine-kanMX6 mid1-GBP-RFP-hphMX6 ade6 leu1-32 ura4-D18 | This study |
| JW5796 | cdc7-GBP-hphMX6 art1-mECitrine-kanMX6 ade6 leu1-32 ura4-D18 | This study |
| JW5798 | art1-mECitrine-kanMX6 rgf3-mCFP-hphMX6 mid1-GBP-hphMX6 ade6 leu1-32 ura4-D18 | This study |
| JW5799 | cdc7-GBP-hphMX6 art1-mECitrine-kanMX6 rgf3-mCFP-hphMX6 ade6 leu1-32 ura4-D18 | This study |
| JW5857 | leu1::kanMX6-P3nmt1-pkc1(HR1-C2)-mECitrine ade6 leu1-32 | This study |
| JW5858 | cdc7-GBP-hphMX6 art1-mECitrine-kanMX6 rlc1-mCFP-kanMX6 ade6 leu1-32 ura4-D18 | This study |
| JW5859 | art1-mECitrine-kanMX6 rlc1-mCFP-kanMX6 ade6 leu1-32 ura4-D18 | This study |
| JW5877 | art1Δ::kanMX6 leu1::kanMX6-P3nmt1-pkc1(HR1-C2)-mECitrine ade6-M210 leu1-32 ura4-D18 | This study |
| JW6165 | rgf3-s44-F3 leu1::kanMX6-P3nmt1-pkc1(HR1-C2)-mECitrine ade6-M210 leu1-32 | This study |
| JW6664 | leu1::kanMX6-P3nmt1-pkc1(HR1-C2)-mECitrine gef2Δ::kanMX4 ade6 leu1-32 ura4-D18 | This study |
| JW6685 | kanMX6-Pmyo2-mEGFP-myo2 art1Δ::kanMX6 sad1-mCherry-natMX6 ade6-M210 leu1-32 ura4-D18 | This study |
| JW6686 | bgs1Δ::ura4+ Pbgs1-GFP-bgs1-leu1+ art1∆::kanMX6 leu1-32 ura4-D18 | This study |
| JW6696 | leu1::kanMX6-P3nmt1-pkc1(HR1-C2)-mECitrine gef3Δ::kanMX6 ade6-M210 leu1-32 ura4-D18 | This study |
| JW6697 | rgf1-∆1::kanMX6 leu1::kanMX6-P3nmt1-pkc1(HR1-C2)-mECitrine ade6 leu1-32 ura4-D18 | This study |
| JW6698 | leu1::kanMX6-P3nmt1-pkc1(HR1-C2)-mECitrine rgf2-Δ1::kanMX6 ade6 leu1-32 ura4-D18 | This study |
| JW6716 | kanMX6-Pmyo2-mEGFP-myo2 sad1-mCherry-natMX6 ade6-M210 leu1-32 ura4-D18 | This study |