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. 2015 Feb 1;26(3):495–505. doi: 10.1091/mbc.E14-10-1477

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© 2015 Sweeney et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Chimera analysis reveals that WAVE1 inhibitory effects on filament elongation stem from its WH2 (V) domain. (A) TIRF analysis of mean elongation rates for filaments polymerized in the presence of GST-VCA chimeras between bovine N-WASP and human WAVE1. Reactions contained 1 μM G-actin (10% Oregon green labeled), 3 μM profilin, and 0 or 100 nM GST-VCA. From 11 to 15 filaments per reaction were analyzed for each of three independent trials. Error bars, SEM. *p <0.01 by one-way ANOVA and Tukey's HSD tests. (B) TIRF analysis of mean elongation rates for filaments polymerized in the presence of GST-VCA chimeras between human WAVE1 and human WAVE2. Reactions were performed and analyzed as in A.