FIGURE 1:

PLD1 expression is down-regulated in diseased muscle and up-regulated during myoblast differentiation and fusion. (A) Reduction of PLD1 mRNA expression in the diaphragm of 8-wk-old mdx5cv mice (n = 5–6, *p < 0.05) but not in gastrocnemius (GA) and tibialis anterior (TA) muscles. (B) PLD1 gene expression was temporarily up-regulated in mouse CTX-injured TA muscle (n = 3, *p < 0.05). (C, E) Mouse C2C12 and (D, F) rat L6 myoblasts were grown to confluence, induced to differentiate for up to 6 d, and analyzed by quantitative RT-PCR (C, D) and Western blotting (E, F) for PLD1 expression. Results are shown as mean ± SD (n = 3). Asterisks indicate statistically significant differences between cells collected at day 0 and the subsequent time points (*p < 0.05; **p < 0.01).