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. 2015 Feb 1;26(3):506–517. doi: 10.1091/mbc.E14-03-0802

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© 2015 Teng et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — PA production on the plasma membrane is required for myocytes to fuse with nascent myotubes. (A) Localization of GFP-PABD and mutant GFP-PABD in proliferating L6 myoblasts. (B) At day 2 of differentiation, GFP-PABD was detected on the PM of mononuclear myocytes (arrows) but inside myotubes (arrowhead). In PLD1-shRNA culture, fewer myocytes with cell surface localization of GFP-PABD were detected. (C) Quantification of PA sensor (GFP-PABD) on the PM of Luc-shRNA and PLD1-shRNA cells (n = 3, *p < 0.05).

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