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. 2015 Feb 1;26(3):530–536. doi: 10.1091/mbc.E14-09-1368

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© 2015 Sadler et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Selective VAMP isoforms are up-regulated during differentiation of 3T3-L1 adipocytes. (A) Equal numbers of fibroblast and adipocyte cells, quantified using propidium iodide staining of nuclei, were homogenized and subjected to centrifugation at 95,000 × g for 1 h to pellet to the total membranes as described. Samples representing equal numbers of cells from day 0 (fibroblasts; d0) or day 8 (adipocytes; d8) were then subjected to SDS–PAGE, followed by immunoblotting with anti-GLUT4 and anti-VAMP antibodies as indicated. Three batches of membranes are displayed on each gel (A–C); d0, fibroblast. (B) The changes in VAMP levels upon differentiation were quantified. Values represent the means of three separate experiments in which fold changes compared with fibroblasts are shown for each VAMP member; values were compared using Student's t test, *p < 0.05.