TABLE 2:
Strains and plasmids used in this study.
| Strain or plasmid | Descriptive name | Genotype or description | Reference or source |
|---|---|---|---|
| Strains | |||
| SEY6210 | WT | MATα leu2-3112 ura3-52 his3-∆200 trp1-∆901 lys2-801 suc2-∆9 GAL | Robinson et al. (1988) |
| KFY7 | vps36∆vps20∆ | SEY6210.1, VPS36::URA3, VPS20::HIS3 | This study |
| SKY8 | vps23∆vps36∆vps20∆ | SEY6210, VPS23::HIS3, VPS36::URA3, VPS20::G418 | This study |
| MBY30 | vps36∆ | SEY6210, VPS30::HIS3 | Babst et al. (2002b) |
| SKY1 | vps23∆vps36∆ | SEY6210, VPS23::HIS3, VPS36::URA3 | This study |
| MBY21 | vps27∆ | SEY6210, VPS27::HIS3 | Odorizzi et al. (1998) |
| EEY6-2 | vps23∆ | SEY6210, VPS23::HIS3 | Babst et al. (2000) |
| MBY3 | vps4∆ | SEY6210, VPS4::TRP1 | Babst et al. (1997) |
| EEY2-1 | vps20∆ | SEY6210, VPS20::HIS3 | Babst et al. (2002a) |
| SKY15 | vps23∆vps36∆snf7∆ | SEY6210, VPS23::HIS3, VPS36::URA3, SNF7::G418 | This study |
| SKY14 | vps23∆vps36∆vps27∆ | SEY6210, VPS23::HIS3, VPS36::URA3, VPS27::G418 | This study |
| MCY23 | vps28∆ | SEY6210, VPS28::HIS3 | Curtiss et al. (2007) |
| DKY48 | vps37∆ | SEY6210, VPS37::HIS3 | Katzmann et al. (2001) |
| MBY65 | mvb12∆ | SEY6210, MVB12::HIS3 | Curtiss et al. (2007) |
| MBY31 | vps22∆ | SEY6210.1, VPS22::HIS3 | Babst et al. (2002b) |
| BWY101 | vps25∆ | SEY6210, VPS25::HIS3 | Babst et al. (2002b) |
| EEY2-1 | vps20∆ | SEY6210, VPS20::HIS3 | Babst et al. (2002a) |
| EEY9 | snf7∆ | SEY6210, SNF7::HIS3 | Babst et al. (2002a) |
| BWY102 | vps24∆ | SEY6210, VPS24::HIS3 | Babst et al. (1998) |
| MBY28 | vps2∆ | SEY6210, VPS2::HIS3 | Babst et al. (2002a) |
| Plasmids | |||
| pSK212 | VPS36-V5-EGFP | VPS36-V5-EGFP (pRS414) | This study |
| pSK243 | vps36(∆PI3P)-V5-GFP | vps36(K38E,R89A,R261E)-V5-EGFP (pRS414) | This study |
| pSK253 | vps36(R89A,R261A)-V5-GFP | vps36(R89A,R261A)-V5-EGFP (pRS414) | This study |
| pSK239 | vps36(∆Ub)-V5-GFP | vps36(T187G,F188A)-V5-EGFP (pRS414) | This study |
| pSK240 | vps36(∆Ub∆PI3P)-V5-GFP | vps36(K38E,R89A,R261E,T187G,F188A)-V5-EGFP (pRS414) | This study |
| pSK241 | vps36(S)-V5-GFP | vps36(F492S)-V5-EGFP (pRS414) | This study |
| pSK215 | vps36(∆GLUE)-V5-GFP | vps36(∆1-289)-V5-EGFP (pRS414) | This study |
| pMB187 | vps4(E233Q) | vps4(E233Q) (pRS415) | Babst et al. (1998) |
| pSK179 | VPS36-V5 | VPS36-V5 (pRS414) | This study |
| pSK213 | vps36(∆PI3P) | vps36(K38E,R89A,R261E)-V5 (pRS414) | This study |
| pSK250 | vps36(R89A,R261A) | vps36(R89A,R261A)-V5 (pRS414) | This study |
| pSK183 | vps36(∆Ub) | vps36(T187G,F188A)-V5 (pRS414) | This study |
| pSK222 | vps36(∆Ub∆PI3P) | vps36(K38E,R89A,R261E,T187G,F188A)-V5 (pRS414) | This study |
| pSD2 | GFP-Cps1 | GFP-Cps1 (pRS415) | Odorizzi et al. (1998) |
| pMB131 | VPS36 | VPS36 (pRS414) | Alam et al. (2004) |
| pSK163 | vps36(S) | vps36(F492S) (pRS414) | This study |
| pSK180 | vps36(S)-V5 | vps36(F492S)-V5 (pRS414) | This study |
| pMB196 | CFP-Cps1 | CFP-Cps1 (pRS425) | This study |
| pSK143 | p2μ[EII-V5] | VPS36-V5, VPS22-HA, VPS25 (pRS425) | This study |
| pMB175 | p2μ[EII] | VPS36, VPS22-HA, VPS25 (pRS425) | Babst et al. (2002b) |
| pSK165 | p2μ[EII(∆GLUE)] | vps36(∆1-289), VPS22-HA, VPS25 (pRS425) | This study |
| pSK223 | p2μ[EII(∆GLUE,S)] | vps36(∆1-289,F492S)-V5, VPS22-HA, VPS25 (pRS425) | This study |
| pSK197 | vps36(∆15) | vps36(∆301-315)-V5 (pRS414) | This study |
| pSK200 | vps36(∆25) | vps36(∆301-325) (pRS414) | This study |
| pSK147 | p2μ[EII(S)] | vps36(F492S)-V5, VPS22-HA, VPS25 (pRS425) | This study |