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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Mech Ageing Dev. 2014 Sep 26;0:35–45. doi: 10.1016/j.mad.2014.09.002

Figure 1. TRAP1 regulates ROS levels in vivo.

Figure 1

(A–C) Optic lobes of young adult Drosophila brains stained with Mitosox, and (D–F) larval segmental nerves of corresponding genotypes stained with antibody against mitochondrial Complex V. (A and D)w1118; +/+; +/+(B and E)w; TRAPΔ4/TRAPΔ4; +/+, (C and F) w; +/+; UAS-TRAP17M/ActinGal4. (G) MitoSox staining is significantly increased in w; TRAPΔ4/TRAPΔ4; +/+ mutants. While staining is marginally reduced in overexpression flies w; +/+; UAS-TRAP17M/ActinGal4, it is not significantly different from wildtype. Error bars denote standard deviation of means. N = 3–5. (**) indicate p < 0.001 from Student’s t-test. Mitochondrial distribution appears normal in mutant and overexpression animals in segmental nerves of Drosophila larvae. In all cases, scale bars equal 10 µm.