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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Mech Ageing Dev. 2014 Sep 26;0:35–45. doi: 10.1016/j.mad.2014.09.002

Figure 2. TRAP1 regulates oxidative stress resistance in young and old Drosophila males.

Figure 2

(A–E) Survival curves of young flies (5 days) of indicated genotypes exposed to 20 mM paraquat. (A) Median survival of male w; TRAP1Δ4/ TRAP1Δ4; +/+ (18.5 hrs ± 0.9) is significantly less than that of w1118; +/+; +/+ (28.4 hrs ± 1.6; p<0.0001). (B) Median survival of male w; +/+; UAS-TRAP17M/ActinGal4 (63.2 hrs ± 4.8) is significantly more than that of w; +/+; UAS-TRAP17M/ + (43.9 hrs ± 3.2; p=0.0002), and that of w; +/+; ActinGal4 (36.2 ± 2.8; p<0.0001). (C) Median survival of female w; TRAP 1A4/TRΔP 1Δ4; +/+ (20.3 hrs ± 1.4) is comparable to that of w1118; +/+; +/+ (22.4 hrs ± 1.4; p=0.4). (D) Median survival of female w; +/+; UAS-TRAP17M/ActinGal4 (43.9 hrs ± 3.3) is significantly higher than that of w; +/+; ActinGal4/+ (32.4 hrs ± 2.2; p=0.004) but comparable to that of w; +/+; UAS-TRAP17M/ + (36.9 hrs ± 2.7; p=0.1). (E) Median survival of young w; TRAP 1A4/TRAP 1A4; UAS-TRAP17M/ActinGal4 males (25.26 hrs ± 1.88) is significantly higher than w; TRAP 1Δ4/TRAP 1Δ4; +/+ (15 hrs ± 0.4; p<0.0001). (F) Median survival is increased in old (40 days) w; +/+; UAS-TRAP17M/ GS-tub5Gal4 males maintained on 5 mM RU-486 (41.47 hrs ± 2.99), relative to control flies of the same genotype, sex and age maintained on vehicle (32.88 ± 1.78; p = 0.03). In all cases, errors denote standard deviation. Statistical significance was determined using the standard chi-squared based log-rank test.