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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Clin Exp Metastasis. 2014 Nov 15;32(1):29–38. doi: 10.1007/s10585-014-9688-8

Table 2.

HER2 expression and amplification in carcinosarcoma cell lines.

Patient qRT-PCR Flow Cytometry IHC FISH
mRNA copy number MFI Cell/tissue block
SARARK-1 27.9 17.2 0 Not Amplified
SARARK-3 75.06 22.8 0 NT*
SARARK-4 36.21 32.36 0 Not Amplified
SARARK-6 5634.2 508.91 3+ Amplified
SARARK-7 61.16 38.35 1+ Not Amplified
SARARK-8 65.35 12.06 0 Not Amplified
SARARK-9 897.64 300.74 3+ Amplified
SARARK-10 10.9 23.05 1+ NT

Abbreviations: qRT-PCR, real time PCR; MFI, mean fluorescence intensity; IHC, immunohistochemistry; FISH, fluorescent in situ hybridization;

*

NT, not tested.