Figure 2. Traditional modes of cell separation currently used in the laboratory and clinic.

(a) The major modes of centrifugation include differential centrifugation, rate-zonal centrifugation, and equilibrium centrifugation. (b) Affinity chromatography allow for separation of a target cell(s) from heterogeneous cell slurry via interactions with a porous matrix conjugated with capture ligands. Adapted from (Kumar and Srivastava, 2010). (c) Traditional set-up of a FACS instrument where cells labeled with fluorescent tags can be separated form un-labeled cell populations. Adapted from (Kang et al., 2011).