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. Author manuscript; available in PMC: 2015 Jan 30.
Published in final edited form as: Eur J Immunol. 2012 Aug 15;42(11):2913–2924. doi: 10.1002/eji.201242504

Figure 5. Previous influenza vaccination does not affect magnitude or cytokine-secreting profile of cross-reactive T-cell memory responses to pH1N1.

Figure 5

(A–C) Magnitude of ex vivo PBMC responses from the IFN-γ only, IL-2 only and IFN-γ/IL-2 dual-secreting subsets to overlapping peptide pools of (A) PB1, (B) M1, and (C) NP of pH1N1 (A/California/04/09) virus, (D) pH1N1 live virus or (E) pH1N1 vaccine strain in pH1N1 sero-negative individuals among those who reported having the 2008 seasonal influenza vaccine (n = 14) and those who did not have the vaccine (n = 17) as determined by fluorescence immunospot. Bars show mean responses with standard error of the mean. Pie charts represent mean proportions of cytokine-secreting responses.