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Opsonophagocytic-killing assay activity of HL-60 cells during differentiation. HL-60 cells were incubated with or without DMF (0.8%), ATRA (1 µM), or VitD3 (20 µg/mL) for the indicated time period. The HL-60 cells were applied to a conventional pneumococcal OPKA against serotype 19F as described in the Materials and Methods. DMF, dimethylformamide; ATRA, all-trans retinoic acid; VitD3, vitamin D3; 7-ADD, 7-aminoactinomycin D.
