Table 2.
Summary of the Candidate Reference Genes and Function
| Gene Symbol | Gene Name | Gene Function | Assay ID* | Amplicon |
|---|---|---|---|---|
| ACTB | Actin, βActin, β | Structural protein (cytoskeletal) | Hs01060665_g1 | 63 |
| ACTR3 | ARP3 actin-related protin 3 homolog | Major constituent of the ARP2/3 complex | Hs01029161_m1 | 72 |
| B2M | β-2-microglobulin | Beta-chain of major histocompatibility complex class I molecules | Hs00984230_m1 | 80 |
| CALM2 | Calmodulin 2 | Calcium-binding messenger protein | ** | 72 |
| CHCHD1 | Coiled-coil-helix domain containing 1 | Nuclear protein | Hs00415054_m1 | 79 |
| GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | Glycolysis | Hs02758991_g1 | 97 |
| GUSB | Glucuronidase, β | Degradation of dermatan and keratan sulfates | Part no 4333767F | 81 |
| HMBS | Hydroxymethylbilane synthase | Heme synthesis, porphyrin metabolism | Hs00609293_g1 | 62 |
| HPRT1 | Hypozanthine phosphoribosyl transferase | Purine salvage | Part no 4333768T | 100 |
| IPO8 | Importin 8 | Nuclear protein import | Hs00183533_m1 | 71 |
| MRPL19 | Mitochondrial ribosomal protein L19 | Component of the large ribosomal subunit | Hs00608519_m1 | 72 |
| NDFIP1 | Nedd4 family interacting protein 1 | Integral Golgi membrane proteins | Hs00228968_m1 | 67 |
| POLR2A | RNA polymerase II | Catalyzes the RNA synthesis | Hs01108291_m1 | 86 |
| PPIA | Peptidylpropyl isomerase A | cis-trans isomerization of proline imidic peptid bonds | Part no. 4333763F | 98 |
| PSMC4 | Proteasome 26S subunit | ATP-dependent degradation of ubiquitinated protien | Hs00197826_m1 | 83 |
| PUM1 | Pumilio homolog 1 | translational regulation of mRNA | Hs00472881_m1 | 77 |
| RPL37A | Ribosomal protein L37a | Protein synthesis | *** | 65 |
| RPLP0 | Ribosomal protein, large, P0 | Structural protein of ribosomes | Hs00420895_gH | 76 |
| rRNA18s | 18S ribosomal RNA | Structural RNA for the small component of ribosomes | Hs03928990_g1 | 61 |
| SF3A1 | Splicing factor 3a, subunit 1 | pre-mRNA splicing | Hs01066327_m1 | 64 |
| TBP | TATA box binding protin | RNA prolymerase II transcription factor | Hs00427621_m1 | 65 |
| TFRC | Transferrin receptor | Uptake of iron | Hs00951083_m1 | 66 |
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Supplied by ABI.
⁎⁎
Primer (forward/reverse) GAGCGAGCTGAGTGGTTGTG/AGTCAGTTGGTCAGCCATGCT probe: TCGCGTCTCGGAAACCGGAGC, supplied by DNA Technology.
⁎⁎⁎
Primer (forward/reverse) TGTGGTTCCTGCATGAAGACA/GTGACAGCGGAAGTGGTATTGTAC probe:TGGCTGGCGGTGCCTGGA, supplied by DNA Technology.