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. 2014 Dec 10;7(6):800–811. doi: 10.1016/j.tranon.2014.09.005

Figure 7.

Figure 7

SIX2 effects on canonical WNT reporter assay activation. (A) TOPFlash activation in HEK-293 cells stably transfected with this reporter plasmid. Baseline control HEK-293 cells having the TOPFlash reporter but not transfected with either vGFP or vGFP-2A-SIX2 plasmid and not stimulated with WNT3a (L) show low activation. When transfected with either control or SIX2 plasmid, and stimulated with WNT3a, canonical WNT activation appears balanced. (B) After transfection of the TOPFlash reporter into the respective model cell lines, an insufficient number of observations were made to conclude statistically significant differences, although 8 of 11 (72.7%) experiments showed repression of reporter activation versus 3 of 11 (27.3%) that showed enhanced activation. (C) To examine whether SIX2 promoted enhanced WiT49 cell survival through CBP/β-catenin–dependent interaction, a survivin reporter assay was transfected into the model cell lines. Notably, SIX2 significantly enhanced canonical WNT pathway activation through the survivin promoter, which represents a pathway to maintain stemness and cell survival.