Table 2.
Histologic Assessment of Proliferative and Apoptotic Activity of Tumor Cells. Histologic Assessment Was Performed for the Tumors Collected at the End of the Treatment. Results Are Shown as Fold Changes of Mean for Each Group in Comparison with Control. Arrows Indicate an Increase (Arrow Up) or Decrease (Arrow Down) of Proliferative or Apoptotic Activity in Treated Tumors versus Respective Controls
| All Models | SW872 | UZLX-STS3 | UZLX-STS5 | |
|---|---|---|---|---|
| Proliferative activity | ||||
| H&E | ||||
| DOX | ↓1.3⁎ | ↓1.3⁎ | ↓1.7⁎ | 1.0 |
| PAZ | ↓2.2⁎⁎## | ↓2.1⁎⁎## | ↓2.5⁎⁎# | ↓1.7⁎# |
| PAZ + DOX | ↓2.0⁎⁎## | ↓1.8⁎⁎## | ↓2.3⁎⁎# | ↓2.4⁎## |
| pHH3 | ||||
| DOX | ↓1.2 | ↑1.1 | ↓1.5⁎ | ↓1.2⁎ |
| PAZ | ↓1.9⁎⁎# | ↓2.0⁎⁎## | ↓1.8⁎ | ↓1.6⁎ |
| PAZ + DOX | ↓1.9⁎# | ↓2.0⁎⁎## | ↓1.9⁎ | ↓1.2 |
| Ki67 | ||||
| DOX | 1.0 | 1.0 | ↑1.2 | 1.0 |
| PAZ | ↓1.2⁎# | ↓1.2⁎ | ↓1.2# | ↓1.2 |
| PAZ + DOX | ↓1.5⁎⁎## | ↓1.3⁎⁎# | ↓2.7⁎⁎## | 1.0 |
| Apoptotic activity | ||||
| H&E | ||||
| DOX | ↑1.1 | 1.0 | ↑1.7⁎ | 1.0 |
| PAZ | ↑1.4 | ↑1.4⁎ | ↑2.2⁎⁎ | 1.0 |
| PAZ + DOX | ↑1.6⁎ | ↑1.3 | ↑2.2⁎⁎ | ↑3.0⁎ |
| CC3 | ||||
| DOX | ↑1.1 | 1.0 | ↑1.3 | ↑2.0 |
| PAZ | ↑1.3 | ↑1.1 | ↑1.7⁎⁎ | 1.0 |
| PAZ + DOX | ↑1.3 | 1.0 | ↑1.6⁎⁎ | ↑4.0⁎ |
Statistical significance was calculated using Mann-Whitney U test.
Compared to control: *P < .05, **P < .005; compared to DOX: #P < .05, ##P < .005.
H&E, hematoxylin and eosin staining; pHH3, phospho-histone H3 immunostaining; CC3, cleaved caspase-3 immunostaining.