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. 2014 Dec 10;7(6):672–680. doi: 10.1016/j.tranon.2014.09.003

Figure 4.

Figure 4

miRNA-ISH of miR-150 and let-7a. Representative microscopic pictures (40-fold magnification) of H&E staining and miRNA-ISH on paraffin-embedded tissue sections (miRNA is stained blue). Left column (A, C, E): non-tumorous liver cells (LP) and surrounding fibrotic tissue (LC); right column (B, D, F): non-tumorous liver (LP) with fibrotic tissue (LC) and HCC (TC) with surrounding tumor capsule (TC). (A) H&E staining of LP and LC; (B) H&E staining of TP and TC; (C) miRNA-ISH for miR-150 demonstrating high expression in LC compared to LP; (D) miRNA-ISH for miR-150 demonstrating high expression in LC and TC and higher expression in TP compared to LP; (E) miRNA-ISH for let-7a demonstrating strongly increased expression in LC compared to LP; (F) miRNA-ISH for let-7a demonstrating high general expression in the two fibrotic tissue compartments with stronger expression in LC versus TC and no difference between LP and TP. Microscope: Nikon Eclipse E1000. Camera: Nikon DS-Ri 1.