Table 1.

Spontaneous release of serotonin, NGF and BDNF from platelets and effects of calcium.

	Serotonin	p1	p2	NGF	p1	p2	BDNF	p1	p2
total	747.2±215 (17)			254.7±48.9 (9)			417.7±99.9 (15)
	[100±28.7 (17)]			[100± 19.2 (9)]			[100±23.9 (15)]
control/10 min	75.5±0.3 (14)	–	–	230.2±52.7 (9)	–	–	55.1 ±12.9 (12)	–	–
	[10.1 ±2.4 (14)]			[90.4± 20.7 (9)]			[13.2 ±3.1 (12)]
control + Ca2+/10 min	192.0±0.01 (9)	ns	–	248.6±32.6 (9)	ns	–	301.6±45.1 (9)	***	–
	[25.7±8.4 (9)]			[97.6± 12.8 (9)]			[72.2±10.8 (9)]
control/60 min	38.1 ±9.0 (12)	–	ns	227.4±32.1 (9)	–	ns	66.4± 12.5 (12)	–	ns
	[5.1 ±1.2(12)]			[89.3± 12.6 (9)]			[15.9±3.0 (12)]
control + Ca2+/60 min	120.3±36.6 (7)	ns	ns	122.5± 19.4 (10)	**	**	397.6±51.0 (9)	***	ns
	[16.1 ±4.9 (7)]			[48.1 ±7.6 (10)]			[95.2±12.2 (9)]

Rat platelets were isolated from whole blood. Platelets were incubated with or without 2 mM Ca²⁺ at 37 °C for 10 or 60 min. Totals and supernatants were collected and serotonin, NGF and BDNF release was analyzed by HPLC-EC and ELISA, respectively. Values are given as mean ± SEM ng/ml/10⁸ platelets (serotonin) or pg/ml/10⁸ platelets (NGF, BDNF). Values in brackets give the mean ± SEM % of total control. The number of analyzed samples is given in parenthesis. Statistical analysis was performed by one-way ANOVA with a Fisher LSD posthoc test. p₁ = control vs. control + Ca²⁺; p₂ = 10 min vs. 60 min;

^*p < 0.05

^**p < 0.01

^***p< 0.001; ns, not significant