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. 2015 Feb 1;128(3):527–540. doi: 10.1242/jcs.157537

Fig. 5.

Fig. 5.

WDR62 recruits JNK1 to the spindle pole. (A) GFP-tagged JNK1 or JNK2 were coexpressed with mCherry-tagged WDR62 or histone 2B (H2B), as a negative control. The cytoplasmic or nuclear localization of JNK isoforms was evaluated in interphase. (B) Nuclear and cytoplasmic fluorescence of GFP-tagged JNK1 or JNK2, in the presence or absence of WDR62, was quantified and expressed as the nuclear to cytoplasmic ratio (Fn/c). Values ∼1 indicate nuclear and cytoplasmic localization, whereas values <1 indicate predominantly cytoplasmic distribution. Data show the mean±s.d. (25 cells evaluated in three independent analyses); *P<0.05; ns, non-significant (Student's t-test). (C) Intracellular localization of GFP-tagged JNK1 or JNK2, with H2B–mCherry, in mitotic cells. Arrows indicate JNK1–GFP localized to spindle poles. (D) The localization of fluorescent-protein-tagged JNK1 coexpressed with WDR62 was determined in mitotic cells. Fluorescent-protein-tagged JNK1 coexpressed with GFP or H2B–mCherry served as negative controls. (E) The localization of fluorescent-protein-tagged JNK2 coexpressed with WDR62 was determined in mitotic cells. (F) Colocalization of JNK1–mCherry and WDR62 T1053A–GFP on interphase microtubules and (G) mitotic spindle poles. (G,H) JNK1–mCherry does not associate with interphase (H) or spindle (I) microtubules when coexpressed with GFP-tagged WDR62-N (amino acids 1–841). Scale bars: 20 µm.