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. Author manuscript; available in PMC: 2015 Jan 30.
Published in final edited form as: Pharmacology. 2010 Jun 23;86(1):15–21. doi: 10.1159/000314333

Fig. 2.

Fig. 2

Effects of cholesterol and 24S-OH Chol on ChAT in axotomized cholinergic brain slices (assay 1). Brain slices of the nBM were cultured without (−; ■) or with 10 ng/ml NGF (●) or with 0.02, 0.2, or 2 μg/ml cholesterol (□) (A, B) or 24S-OH Chol (▲) (C, D). After 2 weeks the slices were fixed and immunohistochemically stained against ChAT. The number of ChAT-positive neurons was counted in whole slices. Statistical analysis was performed by one-way ANOVA with a Fisher PLSD post hoc test. ***p < 0.001; *p < 0.05; NS = not significant.