Figure 4.

Immunogold localization of PLCζ in ejaculated human sperm. (A) Gold particles targeting PLCζ antigenic sites were observed along the IAM (blue arrows). About half of the gold particles were also observed at short distances associated with the acrosomal content or the perinuclear theca (red arrows). Scale bar 500 nm. (B) Quantification of the localization of 104 gold particles. Blue column corresponds to gold particles located along the IAM and red column to gold particles located in the acrosome or in the nuclear theca. (C) Box plot showing the repartition of the numerical values of the distances measured from particles located either in the acrosome (counted as positive) or in the nuclear theca (counted as negative). Nu, nucleus; Ac, acrosome; IAM, inner acrosomal membrane; PNM, perinuclear theca.