Skip to main content
. 2014 Oct 29;21(2):169–185. doi: 10.1093/molehr/gau099

Figure 6.

Figure 6

DNA of Dpy19l2-dependent globozoospermic sperm is fragmented in mouse and human. (A) DNA fragmentation analysis with terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling (TUNEL) assay. Left, Hoechst and TUNEL stainings in WT sperm. Right, Hoechst and TUNEL stainings in Dpy19l2 KO sperm. The histogram shows the level of TUNEL positive cells in WT and KO sperm (n = 5). There was a significant difference between WT and KO sperm (P < 0.001). Bars represent mean ± SE. (B) Similar experiments with control and DPY19L2-deleted sperm in human. Histogram shows the level of TUNEL positive cells in control (n = 5) and DPY19L2-deleted sperm (n = 3). There was a significant difference between control and DPY19L2-deleted sperm (P = 0.01). Bars represent mean ± SE. (C) Fragmentation of sperm is slightly increased during epididymal transit (n = 2). (D) Gamma H2AX histone is expressed during spermatogenesis in tubule sections from Dpy19l2 KO testis.