Table II.
Medical history, laboratory investigations and ICSI outcomes of two couples with males presenting with type I globozoospermia due to full deletion of the DPY19L2 gene.
| Patient ID | Age male years | Age female years | History/habits | Volume ejaculate [sperm] | Sperm mobility | % of globozoospermia | Genotyping | TUNEL % Positive | AB % positive | CMA3 % positive | Nber MII oocytes (%) | Nber 2 PN embryos (%) | Nber of embryos at D3 | Quality and grade of embryos | Nber of transferred Eo (D3) | Delivery |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| M1 | 30 | 28 | Tobacco | 4.8 ml | 20% a + b | 100% | DPY19L2 deleted homoz | 29 | 81.5 | 50 | 14 | 2 | 2 | 2 | 1 | |
| 5–10 cig/d | 21 M/ml | 20% c | (14.3%) | - 6-cell, 10–30% fragment (G III) | ||||||||||||
| Bricklayer | - 6-cell, 30–50% fragment (G III) | |||||||||||||||
| G1-1 | 37 | 29 | Tobacco | 3.2 ml | 30% a + b | 100% | DPY19L2 deleted homoz | 44 | 79 | ND | 10 | 1 | 1 | 0 | 0 | |
| 15 cig/d | 28 M/ml | (10%) | - 5-cell, 10–30% fragment (G III) | |||||||||||||
| Cannabis 2/D | ||||||||||||||||
| G1-2 | 37 | 29 | Tobacco | 2 ml | 20%a + b | 100% | DPY19L2 deleted homoz | ND | ND | ND | 4 | 0 | 0 | 0 | 0 | |
| 15 cig/d | 30 M/ml | 10%c | (0%) | |||||||||||||
| Cannabis 2/D | ||||||||||||||||
| G1-3 | 38 | 30 | Tobacco | 2.5 ml | 20% a + b | 100% | DPY19L2 deleted homoz | 18 | 79 | 78 | 5 | 0 | 0 | 0 | 0 | |
| 5 cig/d | 20 M/ml | 5%c | (0%) | |||||||||||||
| Cannabis 3/w |
ND: not determined; cig/day: cigarettes per day; TUNEL: terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling; AB: aniline blue; CMA3: chromomycin A3; MII: metaphase II oocytes retrieved; PN: pronuclei.