Figure 11.

BLH6 and KNAT7 Repress REV Promoter Activity in Tobacco.

(A) Diagram of the effector and reporter constructs used for transient expression assays in N. benthamiana.

(B) BLH6 and KNAT7 repress REV promoter activity in vivo. BLH6 or KNAT7 was coexpressed with the GUS reporter gene driven by the REV promoter in tobacco leaves shown in (A). Activation of the REV promoter by BLH6 or KNAT7 was measured by assaying GUS activity 2 h after transfection. The expression level of the GUS reporter gene in the leaves transfected with no effector was used as control and was set to 100. Error bars indicate the standard deviations; n = 9 transfections from three independent replicates.