Figure 2.

Yeast Two-Hybrid Assay of BLH and KNAT7 Interactions.

(A) Assay of AD-KNAT7 interactions with DBD-BLH6 or DBD-BLH7 using two reporter genes, URA3 (assayed in Ura^- media) and LacZ (assayed using X-Gal), with growth controls in Leu^- Trp^- media. MYB75-TT8 interaction was used as a positive control, and DBD-BLH-empty prey vector (AD-) interaction was used as a negative control.

(B) Schematic diagram of KNAT7 with four different fragments used to test interaction with BLH6, KNOX1, KNOX2, MEINOX domain (KNOX1+KNOX2), and homeodomain.

(C) Assay of AD-KNAT7 fragments interaction with DBD-BLH6 using two reporter genes, URA3 (assayed in Ura^- media) and LacZ (assayed on X-Gal), with growth controls in Leu^- Trp^- media. MYB75-TT8 interaction was used as a positive control. DBD-BLH6- empty prey vector (AD-) interaction was used as a negative control, shown in (A).

(D) Schematic diagram of BLH6 with six different fragments: SKY, BELL, MID (longer), MID (shorter), HM, and HM+ VSLTLGL box (Vbox). Truncation scheme of BLH6 is shown in detail below.

(E) Assay of DBD-BLH6 fragments interaction with AD-KNAT7 and empty prey vector (AD-Empty) using two reporter genes, URA3 (assayed in Ura^- media) and LacZ (assayed on X-Gal), with growth controls in Leu^- Trp^- media. MYB75-TT8 interaction was used as a positive control, and DBD-BLH6- empty prey vector (AD-) interaction was used as a negative control.

[See online article for color version of this figure.]