Figure 5.

Triple car Mutants Show Reduced Sensitivity to ABA-Mediated Inhibition of Seedling Establishment and Root Growth.

(A) Quantification of ABA-mediated inhibition of seedling establishment of the Col wild type compared with single, double, and triple car mutants. Approximately 100 seeds of each genotype were sown on each plate and scored for the presence of green expanded cotyledons 5 d later. The letters denote significant differences among the different genetic backgrounds (P < 0.05, Fisher’s least significant difference test).

(B) Photographs of the Col wild type and triple car mutants grown for 7 d on MS medium either lacking or supplemented with 1 μM ABA.

(C) Quantification of ABA-mediated root growth inhibition of the Col wild type compared with single, double, and triple car mutants. Asterisks indicate P < 0.05 (Student’s t test) when comparing data of car mutants with Col wild-type plants in the same assay conditions.

(D) Photographs of representative seedlings 10 d after the transfer of 4-d-old triple car mutants seedlings from MS medium to plates lacking or supplemented with 10 μM ABA.

[See online article for color version of this figure.]