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. 2014 Dec 30;26(12):4782–4801. doi: 10.1105/tpc.114.131607

Figure 2.

Figure 2.

The Expression Pattern of miR172c.

(A) and (B) qRT-PCR relative expression of miR172c in roots. Seven-day-old seedlings were inoculated with B. japonicum strain USDA110, and roots were harvested at 0, 1, 3, 6, 12, and 24 h after inoculation (HAI) (A) or 0, 1, 3, 5, and 10 DAI (B) (n = 5). miR1520d was used as an endogenous control for gene expression. Expression levels are shown as means ± se from three replicates. Asterisks represent statistically significant differences (Student’s t test, ***P < 0.001, *P < 0.05).

(C) qRT-PCR of miR172c in nodules. Seven-day-old seedlings were inoculated with B. japonicum strain USDA110, and nodules were harvested at 10, 14, 21, and 28 DAI. Asterisks represent statistically significant differences (Student’s t test, ***P < 0.001).

(D) and (E) miR172c histochemical analysis of promiR172c:GUS during nodule initiation. Bars = 200 μm.

(F) to (H) GUS staining of promiR172c:GUS in 10-d-old (F), 14-d-old (G), and 28-d-old (H) nodules. Bars in (F) = 100 μm; bars in (G) = 250 μm; bars in (H) = 400 μm.