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. 2014 Dec 30;26(12):4782–4801. doi: 10.1105/tpc.114.131607

Figure 5.

Figure 5.

The Expression Pattern of the miR172c Target Gene NNC1.

(A) and (B) qRT-PCR of NNC1 in roots at various times after B. japonicum inoculation during early infection (A) and nodule development (B) (n = 5). Transcript levels were normalized to the expression of GmELF1b in each sample. Expression levels are shown as means ± se from three replicates. Asterisks represent statistically significant differences (Student’s t test, ***P < 0.001). HAI, h after inoculation.

(C) Expression of proGmNNC1:GUS at the nodule initiation stage at 10 DAI. Bar = 50 μm.

(D) to (F) Expression of proGmNNC1:GUS in developing nodules at 10 (D), 14 (E), or 28 (F) DAI. Bars in (D) = 100 μm; bars in (E) = 300 μm; bars in (F) = and 400 μm.