Figure 8.

3D Crystal Structure of the PSM from Arabidopsis PhyB.

(A) Ribbon diagram of the PSM with the PAS (blue), GAF (green), and PHY (orange) domains indicated. The knot-lasso is colored yellow, the helical spine is indicated with a dashed red line, and the hairpin is delineated with a bracket. Connectivity of the PHY domain in the disordered regions is illustrated with dashed black lines. The second subunit of the dimer is colored gray. PΦB is colored cyan. N, N terminus.

(B) Bilin and surrounding amino acids. NTE, GAF domain, knot lasso, hairpin, and PΦB carbons are colored blue, green, yellow, orange, and cyan, respectively. Amino acid side chains are labeled. Hydrogen bonds are indicated with black dashed lines. Oxygens, red; nitrogens, blue; and sulfurs, gold. pw, pyrrole water. (Adapted from Burgie et al. [2014a], Figure 2C.)