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. 2014 Dec 16;26(12):4889–4902. doi: 10.1105/tpc.114.132472

Figure 2.

Figure 2.

Somatic HR Frequencies.

(A) Structure of the HR reporter construct of the IC9 line. Two fragments of the GUS gene (GU and US) with homology to each other are separated by a spacer sequence. Through intermolecular recombination events, a functional GUS gene can be restored. All recombined cells and their daughter cells can be quantified by histological staining.

(B) Mean recombination frequencies of the wild type, rtel1-1, and three complemented lines, rtel1-1:RTEL1#1, rtel1-1:RTEL1#2, and rtel1-1:RTEL1#3 (n = 3).

(C) Mean recombination frequencies of the wild type, rtel1-1, fancm-1, and the double mutant rtel1-1 fancm-1 (n = 8).

Columns in (B) and (C) correspond to mean values, and error bars represent sd. Statistical differences were calculated by the Mann-Whitney test: *P < 0.05, **P < 0.01, ***P < 0.001.