Figure 5.

Sensitivity against cis-Platin-Induced DNA Intrastrand CLs.

Fresh weights relative to untreated controls after treatment with 5, 7.5, 8.5, and 10 μM cis-platin are shown.

(A) While there was no difference in sensitivity against cis-platin in fancm-1 compared with the wild type, the single mutant rtel1-1 was more sensitive than the wild type at all tested concentrations. The rtel1-1 fancm-1 double mutant was significantly more sensitive against cis-platin treatment than either of the corresponding single mutants (n = 8).

(B) Both the rtel1-1 and mus81-1 single mutants were more sensitive against cis-platin at all tested concentrations. In contrast with treatment with MMC, after cis-platin treatment the rtel1-1 mus81-1 double mutant line was significantly more sensitive than either of the single mutants at three of the four tested concentrations (n = 8).

Columns correspond to mean values, and error bars represent sd. Statistical differences were calculated by the Mann-Whitney test: *P < 0.05, **P < 0.01, ***P < 0.001.