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. 2014 Dec 23;26(12):4763–4781. doi: 10.1105/tpc.114.132738

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Figure 2. — FRI Localizes to the Cajal Body in the Nucleus.

(A) Subcellular localization of FRI in the leaf epidermal cells (upper panel) and root tip cells (lower panel) of 2-week-old FRI-GFP lines not subjected to vernalization treatment. From left to right: GFP fluorescence, DAPI staining showing the nuclei, bright-field (BF), merged image of GFP and DAPI, and merged image of GFP/DAPI/bright field. Bar = 20 μm.

(B) Colocalization of FRI-GFP and the Cajal body in the leaf epidermal (upper panels) and root tip (lower panels) cells of 2-week-old FRI-GFP Atcoilin-RFP lines not subjected to vernalization treatment. From left to right: RFP fluorescence, DAPI staining showing the nuclei, GFP fluorescence; bright field, merged image of RFP/GFP/DAP, and merged image of RFP/GFP/DAP/bright field. Bar = 20 μm.