Figure 6.

NF-YA2 and NF-YB3 Were Identified as Candidate Components of the Trimer That Interacts with DREB2A under Heat Stress Conditions.

(A) The growth of yeast cells harboring the N-terminal region of DREB2A or full-length DPB3-1 fused to the GAL4 DNA binding domain (BD). SD/-L-W was used as the nonselective medium, and SD-L-W-H-Ade (quadruple drop out [QDO]) was the selective medium. H2B-like proteins were expressed as fusion proteins with the GAL4 activation domain (AD).

(B) Expression patterns of the NF-YB1, 2, 3, 6, and 7 and DPB4 genes under heat and dehydration stress conditions in 14-d-old plants.

(C) The growth of yeast cells harboring NF-YA family proteins fused to the GAL4 DNA binding domain (BD), NF-YB3 protein fused to the GAL4 activation domain (AD), and DPB3-1 fused to a nuclear localization signal (NLS) on selective medium (SD/-L-W-H-M-Ade).

(D) Coimmunoprecipitation of GFP-NF-YA2, Myc-NF-YB3, and FLAG-DPB3-1 in N. benthamiana leaves. Total protein extracts (Input) or immunoprecipitated (IP) fractions using an anti-GFP antibody were analyzed by immunoblotting (IB) using anti-GFP, anti-myc, or anti-FLAG antibodies. The triangles indicate specific signals for each expressed protein.