Figure 1.

Experimental Overview of Transcriptome Analysis Performed and Numbers of Significantly Regulated Genes Identified.

(A) Infection time course of wild-type (A17) M. truncatula root hairs from seedlings inoculated with S. meliloti expressing lacZ. Major developmental milestones of infection: 1 dpi represents preinfection before visible root hair curling, at 3 dpi microcolonies had formed within curled root hairs, and at 5 dpi elongating infection threads were seen. Bar = 20 μm.

(B) Genes upregulated in the wild type 1, 3, and 5 dpi with Sm1021.

(C) Genes upregulated 5 dpi with Sm1021 in the wild type and in the hyperinfected skl mutant.

(D) Genes upregulated in wild-type 1 dpi with Sm1021 and 1 d post-treatment with Nod factor.

(E) Summary of up- and downregulated genes for each treatment. dpt, days post-treatment.

Genes with significant regulation relative to control experiments are shown (>2-fold change; P < 0.05).