Figure 8.

Expression of GH3.1-GUS Promoter Fusion during Rhizobial Infection and Development of Nodules and Lateral Roots.

(A) to (D) ProGH3.1:GUS expression in A. rhizogenes-induced transgenic hairy roots showing expression at infection sites ([A] and [B]) and in nodules ([C] and [D]), 3 weeks after inoculation with S. meliloti carrying pXLGD4 (lacZ).

(E) to (G) ProGH3.1:GUS expression in a lateral root tip (E), a lateral root primordium (F), and an emerging lateral root (G).

(H) ProGH3.1:GUS expression in zone I and zone II in a mature nodule (3 weeks after inoculation with S. meliloti).

The blue color ([A] to [H]) indicates GUS staining, and the magenta color indicates LacZ staining of S. meliloti. Bars = 100 μm.