Figure 7.

Effect of Histone Hyperacetylation on the QC Identity.

Longitudinal optical sections of Arabidopsis stem cell niche in 4-d-old wild type.

(A) Col-0.

(B) Col-0 treated overnight with 100 ng/mL TSA, showing divided QC cells (arrow).

(C) Number of QC divisions per root in 5-d-old seedlings in control (Col-0) and plants treated overnight with TSA. Values represent mean ± se from 20 cells. Student’s t test, **P < 0.01.

(D) Col-0 root expressing H2AZ-GFP.

(E) Col-0 H2AZ-GFP root after TSA treatment showing expression of H2AZ-GFP in QC cells (arrow).

(F) to (I) Roots expressing WOX5:GFP. WOX5:GFP signal in 4-d-old (F) and 5-d-old (G) control roots and in plants treated with TSA for 24 h (H) and 48 h (I), showing a strong decrease in WOX5 signal upon TSA treatment. Bars = 20 μm.