Fig. 2.

The effect of taurine, N-acetyl cysteine (NAC) and 1-bromoheptane (BHP) on amodiaquine-induced cytotoxicity toward isolated rat hepatocytes. Isolated rat hepatocytes (10⁶ cells/mL) were incubated at 37°C in rotating round bottom flasks with 95% O₂ and 5% CO₂ in Krebs-Henseleit buffer (pH 7.4). Data are shown as Mean ± SEM for at least three separate experiments. ^aSignificantly different from control group (n=3, ANOVA, P<0.001). ^bSignificant difference as compared with amodiaquine-treated hepatocytes (n=3, ANOVA, P<0.05). ^cSignificantly different from amodiaquine-treated group (n=3, ANOVA, P<0.05). ^dSignificantly different from glutathione-depleted cells, which were treated with amodiaquine (n=3, ANOVA, P<0.001).