Table 2. Effects of Thapsigargin on Re-Growth of Crossing of Laminin-Positive Capillaries.
Region | Control | Thapsigargin |
---|---|---|
Ctx-nBM | 19 ± 3 (11) - | 38 ± 4 (21) ** |
vMes-Ctx | 6 ± 2 (11) - | 6 ± 2 (9) ns |
vMes-dStr | 10 ± 4 (11) - | 7 ± 3 (9) ns |
nBM-dStr | 5 ± 2 (11) - | 2 ± 1 (9) ns |
dStr-Ctx | n.c. (11) | n.c. (6) |
A 4 week old co-culture brain slice, composed of the basal nucleus of Meynert (nBM), the dorsal striatum (dStr), the cortex (Ctx) and the ventral mesencephalon (vMes), was incubated with 3 μM thapsigargin and then further 3 days without. Then slices were fixed and stained for laminin immunohistochemistry. The number of capillary crossings per cm between the different brain regions was counted under the microscope. Values are given as mean ± S.E.M.; values in parenthesis give the number of analyzed slices. Statistical analysis was performed by one way ANOVA with a Fisher PLSD posthoc test (**p<0.01; ns, not significant; n.c., no crossings).