Fig 3.
NOX4 expression is increased during influenza virus infection.A. Real-time qPCR assay of NOX isoform levels in PR8 virus-infected NCI-H292 cells, normalized to levels in mock-infected cells. NOX2 and NOX4 levels changed over time. Values are mean and SD of three independent experiments (**P < 0.01; ***P < 0.001, Student's t-test).B. Western blot of uninfected (Ctr) and PR8 virus-infected NCI-H292 cells up to 6 h, using antibodies against NOX2 (left panels) and NOX4 (right panels). Actin was used as loading control for densitometric analysis of three independent experiments (bottom).