Table 1.
Summary of de novo assembly of transcriptome sequence reads without reference genome
| Samples | Total raw reads | Total clean reads | Total clean nucleotides (nt) | Q20 percentage | GC percentage | Type of gene detected | Total number | Total length (nt) | Mean length (nt) | N50 |
|---|---|---|---|---|---|---|---|---|---|---|
| RF1 | 48 335 970 | 44 166 280 | 4 416 628 000 | 96,59% | 45,65% | Raw sequences | 68 768 | 32 762 368 | 476 | 1284 |
| Consensus sequences | 41 770 | 37 272 781 | 892 | 1627 | ||||||
| RF2 | 49 872 928 | 45 562 458 | 4 556 245 800 | 96,55% | 45,91% | Raw sequences | 68 357 | 33 018 028 | 483 | 1289 |
| Consensus sequences | 41 881 | 37 472 313 | 895 | 1611 |
First column shows the total raw reads, the next columns shows the clean reads and nucleotids. Q20 percentage column shows the proportion of nucleotides with quality value larger than 20. GC percentage is proportion of guanidine and cytosine nucleotides among total nucleotides. The total number column shows the total sequences obtained, that represents the all raw sequences and consensus sequences, respectively. Next columns show the total nucleotides length and their mean length. The last column shows the N50 value (defined as the length for which the collection of all sequences of that length or longer contains at least half of the sum of the lengths of all sequences).