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. 2015 Jan 30;6:15. doi: 10.3389/fphys.2015.00015

Figure 1.

Figure 1

Western blot of NOX subunit expression in the sternohyoid muscle from sham (S) and chronic intermittent hypoxia (CIH)-exposed rats. (A) Expression of the catalytic superoxide-generating NOX 2 subunit and the co-anchoring p22 phox subunit with the corresponding Ponceau S stained membrane used to normalize protein loading and electro-transfer. MWM = pre-stained molecular weight markers. (B) Expression of the organizer subunit, p47 phox with corresponding Ponceau S stained membrane. Densitometric analysis of NOX subunit band intensities normalized by densitometric intensities of corresponding Ponceau-S stained proteins expressed in arbitrary units (a.u.) are shown in (C–E). (C) A near 3-fold increase in NOX 2 expression was observed in the CIH-exposed group compared to sham control (**P = 0.002; Student unpaired t-test, n = 4 per group). (D) No change in the p22 phox subunit expression was observed (P = 0.884, n = 4 per group). (E) A near 2-fold increase in the p47 phox subunit expression was observed in the CIH-exposed group compared to sham control (*P = 0.014, n = 6 per group). Values are mean ± SEM.