Figure 4.

Correlation of the expression levels of the Lipid Droplets with CD133 and Wnt/β-catenin. (A, B): The expression of the LDs in CD133^High and CD133^Low cells were analyzed by flow cytometry. Cells were stained with an anti-CD133 APC-conjugated and then with BODIPY 493/503. Both CD133^High samples (A and B red lines) have a higher expression of LDs compared to the CD133^Low (black lines). (C): Schematic representation of the TOP-GFP Wnt construct. (D, E): Cells were sorted for GFP expression (GFP^High and GFP^Low) and then stained for LDs with LD540 dye; both TOP-GFP samples have the same behavior showing as Wnt/β-catenin pathway expression clearly correlates with LDs quantity. Abbreviations: APC, allophycocyanin; FACS, fluorescence-activated cell sorting; GFP, green fluorescent protein; TOP, TCF Optimal Promoter (TOP).