(A) Corticosterone concentrations in blood collected 30 min after the last handling bout or injection. Basal corticosterone concentrations were significantly (P < 0.01) higher in Sprague–Dawley rats and decreased with continued handling and saline injections. Concentrations for Lewis rats remained constant across groups. Error bars represent 1 SD. (B) Scatter plot showing individual corticosterone concentrations. Sprague–Dawley rats again showed greater interindividual variability as compared with Lewis rats, although this variability decreased with continued handling. There was no change in Lewis interindividual variability with continued handling. L, Lewis; S, Sprague. NH, no handling; SH, short handling; LH, long handling; SI, saline injection.