Table 4.
Primary antibodies with source and dilution used for detection of target proteins.
Antibody | Source | Catalog number | Dilution |
Caveolin-1 (N20) | Santa Cruz Biotechnology | sc-894 | 1:400 |
Purified mouse Flotillin-1 | BD Transduction | 610820 | 1:100 |
Cdc42 polyclonal | Thermo Scientific | PA5-17544 | 1:1000 |
Monoclonal anti-PIP5K2A | Sigma-Aldrich | WH0005305M1-100UG | 1:350 |
Monoclonal anti-ß-actin | Sigma-Aldrich | A5441-2ML | 1:5000 |
Anti-Dynamin II polyclonal | Thermo Scientific | PA1-661 | 1:1000 |
Antibody Clathrin | Haucke group (FU Berlin, Germany) | unknown | 1:500 |